Coding

Part:BBa_K2905008

Designed by: Brianna Ricker   Group: iGEM19_Alma   (2019-06-24)


PylC

3-methylornithine--L-lysine ligase. Starts with a His-6 tag, followed by a NheI site scar and a TEV protease recognition site, fused in frame to the PylC gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 153
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None